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MiddleBrook Pharmaceuticals solid medium middlebrook 7h11
Solid Medium Middlebrook 7h11, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/solid+medium+middlebrook+7h11/middlebrook+7h9+broth/pmc11694965-45-7-7
Average 90 stars, based on 1 article reviews
solid medium middlebrook 7h11 - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Activity Assay:

Article Title: Comparison of MALDI-TOF MS With HPLC and Nucleic Acid Sequencing for the Identification of Mycobacterium Species in Cultures Using Solid Medium and Broth
Article Snippet: Objectives: The genus Mycobacterium contains over 150 species including pathogenic and nonpathogenic strains.. Accurate species level identification can aid in differentiating environmental contamination from true infection, and also can aid in selection of antimicrobial therapy.. Methods: We evaluated the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the routine identification of clinical isolates of mycobacteria using 2 commercially available spectral reference libraries, and also assessed the impact of mycobacterial culture using solid medium and broth on MALDI-TOF MS–based identification.

Article Title: Azide-alkyne cycloaddition en route towards 1H-1,2,3-triazole-tethered β-lactam-ferrocene and β-lactam-ferrocenylchalcone conjugates: synthesis and in vitro anti-tubercular evaluation.
Article Snippet: A diverse range of triazoles were prepared following well established, Cu-mediated azide–alkyne cycloaddition reactions with the aim of probing the anti-tubercular structure–activity relationships (SAR) within the β-lactam–ferrocene–triazole conjugate family.. The anti-tubercular evaluation studies of the synthesized conjugates revealed that none of the scaffolds exhibited any activity that restricted mycobacterial growth even at high doses.. The introduction of various substituents onto the N-1 of the β-lactam ring, introducing monoor di-ferrocenylchalcone substituents at the C-3 position as well as introducing a spacer of varying chain length failed to produce any significant enhancement in the activity profiles.

Article Title: An attenuated Mycobacterium tuberculosis clinical strain with a defect in ESX-1 secretion induces minimal host immune responses and pathology
Article Snippet: All M.tb strains used in this study were grown at 37 °C for 3–4 weeks either on solid medium (Middlebrook 7H11) or in liquid medium (Middlebrook 7H9) supplemented with 10% (v/v) oleic acid–albumin–dextrose–catalase (OADC).

Article Title: Rifampicin and isoniazid resistance not promote fluoroquinolone resistance in Mycobacterium smegmatis
Article Snippet: Spontaneous FQ-resistant mutant colonies were selected on Middlebrook 7H11 solid medium with 10% OADC (7H11-OADC) and 1.0 μg/mL levofloxacin (2× MIC).

Article Title: Experimental Model of Tuberculosis in the Domestic Goat after Endobronchial Infection with Mycobacterium caprae
Article Snippet: The inoculum was titrated after the inoculation in duplicate by 10-fold serial dilution in Middlebrook 7H11 solid medium; accordingly, each goat received 1.5 × 10 3 CFU of M. caprae .

Article Title: The Mycobacterium tuberculosis β-oxidation genes echA5 and fadB3 are dispensable for growth in vitro and in vivo
Article Snippet: Intracellular survival and growth was assessed by lysis of the monolayers by the addition of water followed by a 30 min incubation at room temperature and enumeration of bacteria by serial dilution in PBS-Tween plating onto Middlebrook 7H11 solid medium supplemented with 0.5% glycerol and 10% OADC.

Article Title: Identification of β-Lactams Active against Mycobacterium tuberculosis by a Consortium of Pharmaceutical Companies and Academic Institutions
Article Snippet: To plate for CFU, lung homogenates were thawed, and bacilli were enumerated on Middlebrook 7H11 solid medium supplemented with 10% OADC and 0.4% activated charcoal for ∼18 days at 37 °C.

Knock-Out:

Article Title: Comparison of MALDI-TOF MS With HPLC and Nucleic Acid Sequencing for the Identification of Mycobacterium Species in Cultures Using Solid Medium and Broth
Article Snippet: Objectives: The genus Mycobacterium contains over 150 species including pathogenic and nonpathogenic strains.. Accurate species level identification can aid in differentiating environmental contamination from true infection, and also can aid in selection of antimicrobial therapy.. Methods: We evaluated the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the routine identification of clinical isolates of mycobacteria using 2 commercially available spectral reference libraries, and also assessed the impact of mycobacterial culture using solid medium and broth on MALDI-TOF MS–based identification.

Article Title: Azide-alkyne cycloaddition en route towards 1H-1,2,3-triazole-tethered β-lactam-ferrocene and β-lactam-ferrocenylchalcone conjugates: synthesis and in vitro anti-tubercular evaluation.
Article Snippet: A diverse range of triazoles were prepared following well established, Cu-mediated azide–alkyne cycloaddition reactions with the aim of probing the anti-tubercular structure–activity relationships (SAR) within the β-lactam–ferrocene–triazole conjugate family.. The anti-tubercular evaluation studies of the synthesized conjugates revealed that none of the scaffolds exhibited any activity that restricted mycobacterial growth even at high doses.. The introduction of various substituents onto the N-1 of the β-lactam ring, introducing monoor di-ferrocenylchalcone substituents at the C-3 position as well as introducing a spacer of varying chain length failed to produce any significant enhancement in the activity profiles.

Article Title: An attenuated Mycobacterium tuberculosis clinical strain with a defect in ESX-1 secretion induces minimal host immune responses and pathology
Article Snippet: All M.tb strains used in this study were grown at 37 °C for 3–4 weeks either on solid medium (Middlebrook 7H11) or in liquid medium (Middlebrook 7H9) supplemented with 10% (v/v) oleic acid–albumin–dextrose–catalase (OADC).

Article Title: Rifampicin and isoniazid resistance not promote fluoroquinolone resistance in Mycobacterium smegmatis
Article Snippet: Spontaneous FQ-resistant mutant colonies were selected on Middlebrook 7H11 solid medium with 10% OADC (7H11-OADC) and 1.0 μg/mL levofloxacin (2× MIC).

Article Title: Experimental Model of Tuberculosis in the Domestic Goat after Endobronchial Infection with Mycobacterium caprae
Article Snippet: The inoculum was titrated after the inoculation in duplicate by 10-fold serial dilution in Middlebrook 7H11 solid medium; accordingly, each goat received 1.5 × 10 3 CFU of M. caprae .

Article Title: The Mycobacterium tuberculosis β-oxidation genes echA5 and fadB3 are dispensable for growth in vitro and in vivo
Article Snippet: Intracellular survival and growth was assessed by lysis of the monolayers by the addition of water followed by a 30 min incubation at room temperature and enumeration of bacteria by serial dilution in PBS-Tween plating onto Middlebrook 7H11 solid medium supplemented with 0.5% glycerol and 10% OADC.

Article Title: Identification of β-Lactams Active against Mycobacterium tuberculosis by a Consortium of Pharmaceutical Companies and Academic Institutions
Article Snippet: To plate for CFU, lung homogenates were thawed, and bacilli were enumerated on Middlebrook 7H11 solid medium supplemented with 10% OADC and 0.4% activated charcoal for ∼18 days at 37 °C.

Infection:

Article Title: Comparison of MALDI-TOF MS With HPLC and Nucleic Acid Sequencing for the Identification of Mycobacterium Species in Cultures Using Solid Medium and Broth
Article Snippet: Objectives: The genus Mycobacterium contains over 150 species including pathogenic and nonpathogenic strains.. Accurate species level identification can aid in differentiating environmental contamination from true infection, and also can aid in selection of antimicrobial therapy.. Methods: We evaluated the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the routine identification of clinical isolates of mycobacteria using 2 commercially available spectral reference libraries, and also assessed the impact of mycobacterial culture using solid medium and broth on MALDI-TOF MS–based identification.

Article Title: Azide-alkyne cycloaddition en route towards 1H-1,2,3-triazole-tethered β-lactam-ferrocene and β-lactam-ferrocenylchalcone conjugates: synthesis and in vitro anti-tubercular evaluation.
Article Snippet: A diverse range of triazoles were prepared following well established, Cu-mediated azide–alkyne cycloaddition reactions with the aim of probing the anti-tubercular structure–activity relationships (SAR) within the β-lactam–ferrocene–triazole conjugate family.. The anti-tubercular evaluation studies of the synthesized conjugates revealed that none of the scaffolds exhibited any activity that restricted mycobacterial growth even at high doses.. The introduction of various substituents onto the N-1 of the β-lactam ring, introducing monoor di-ferrocenylchalcone substituents at the C-3 position as well as introducing a spacer of varying chain length failed to produce any significant enhancement in the activity profiles.

Article Title: An attenuated Mycobacterium tuberculosis clinical strain with a defect in ESX-1 secretion induces minimal host immune responses and pathology
Article Snippet: All M.tb strains used in this study were grown at 37 °C for 3–4 weeks either on solid medium (Middlebrook 7H11) or in liquid medium (Middlebrook 7H9) supplemented with 10% (v/v) oleic acid–albumin–dextrose–catalase (OADC).

Article Title: Rifampicin and isoniazid resistance not promote fluoroquinolone resistance in Mycobacterium smegmatis
Article Snippet: Spontaneous FQ-resistant mutant colonies were selected on Middlebrook 7H11 solid medium with 10% OADC (7H11-OADC) and 1.0 μg/mL levofloxacin (2× MIC).

Article Title: Experimental Model of Tuberculosis in the Domestic Goat after Endobronchial Infection with Mycobacterium caprae
Article Snippet: The inoculum was titrated after the inoculation in duplicate by 10-fold serial dilution in Middlebrook 7H11 solid medium; accordingly, each goat received 1.5 × 10 3 CFU of M. caprae .

Article Title: The Mycobacterium tuberculosis β-oxidation genes echA5 and fadB3 are dispensable for growth in vitro and in vivo
Article Snippet: Intracellular survival and growth was assessed by lysis of the monolayers by the addition of water followed by a 30 min incubation at room temperature and enumeration of bacteria by serial dilution in PBS-Tween plating onto Middlebrook 7H11 solid medium supplemented with 0.5% glycerol and 10% OADC.

Article Title: Identification of β-Lactams Active against Mycobacterium tuberculosis by a Consortium of Pharmaceutical Companies and Academic Institutions
Article Snippet: To plate for CFU, lung homogenates were thawed, and bacilli were enumerated on Middlebrook 7H11 solid medium supplemented with 10% OADC and 0.4% activated charcoal for ∼18 days at 37 °C.

Software:

Article Title: Comparison of MALDI-TOF MS With HPLC and Nucleic Acid Sequencing for the Identification of Mycobacterium Species in Cultures Using Solid Medium and Broth
Article Snippet: Objectives: The genus Mycobacterium contains over 150 species including pathogenic and nonpathogenic strains.. Accurate species level identification can aid in differentiating environmental contamination from true infection, and also can aid in selection of antimicrobial therapy.. Methods: We evaluated the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the routine identification of clinical isolates of mycobacteria using 2 commercially available spectral reference libraries, and also assessed the impact of mycobacterial culture using solid medium and broth on MALDI-TOF MS–based identification.

Article Title: Azide-alkyne cycloaddition en route towards 1H-1,2,3-triazole-tethered β-lactam-ferrocene and β-lactam-ferrocenylchalcone conjugates: synthesis and in vitro anti-tubercular evaluation.
Article Snippet: A diverse range of triazoles were prepared following well established, Cu-mediated azide–alkyne cycloaddition reactions with the aim of probing the anti-tubercular structure–activity relationships (SAR) within the β-lactam–ferrocene–triazole conjugate family.. The anti-tubercular evaluation studies of the synthesized conjugates revealed that none of the scaffolds exhibited any activity that restricted mycobacterial growth even at high doses.. The introduction of various substituents onto the N-1 of the β-lactam ring, introducing monoor di-ferrocenylchalcone substituents at the C-3 position as well as introducing a spacer of varying chain length failed to produce any significant enhancement in the activity profiles.

Article Title: An attenuated Mycobacterium tuberculosis clinical strain with a defect in ESX-1 secretion induces minimal host immune responses and pathology
Article Snippet: All M.tb strains used in this study were grown at 37 °C for 3–4 weeks either on solid medium (Middlebrook 7H11) or in liquid medium (Middlebrook 7H9) supplemented with 10% (v/v) oleic acid–albumin–dextrose–catalase (OADC).

Article Title: Rifampicin and isoniazid resistance not promote fluoroquinolone resistance in Mycobacterium smegmatis
Article Snippet: Spontaneous FQ-resistant mutant colonies were selected on Middlebrook 7H11 solid medium with 10% OADC (7H11-OADC) and 1.0 μg/mL levofloxacin (2× MIC).

Article Title: Experimental Model of Tuberculosis in the Domestic Goat after Endobronchial Infection with Mycobacterium caprae
Article Snippet: The inoculum was titrated after the inoculation in duplicate by 10-fold serial dilution in Middlebrook 7H11 solid medium; accordingly, each goat received 1.5 × 10 3 CFU of M. caprae .

Article Title: The Mycobacterium tuberculosis β-oxidation genes echA5 and fadB3 are dispensable for growth in vitro and in vivo
Article Snippet: Intracellular survival and growth was assessed by lysis of the monolayers by the addition of water followed by a 30 min incubation at room temperature and enumeration of bacteria by serial dilution in PBS-Tween plating onto Middlebrook 7H11 solid medium supplemented with 0.5% glycerol and 10% OADC.

Article Title: Identification of β-Lactams Active against Mycobacterium tuberculosis by a Consortium of Pharmaceutical Companies and Academic Institutions
Article Snippet: To plate for CFU, lung homogenates were thawed, and bacilli were enumerated on Middlebrook 7H11 solid medium supplemented with 10% OADC and 0.4% activated charcoal for ∼18 days at 37 °C.

Cell Culture:

Article Title: Comparison of MALDI-TOF MS With HPLC and Nucleic Acid Sequencing for the Identification of Mycobacterium Species in Cultures Using Solid Medium and Broth
Article Snippet: Objectives: The genus Mycobacterium contains over 150 species including pathogenic and nonpathogenic strains.. Accurate species level identification can aid in differentiating environmental contamination from true infection, and also can aid in selection of antimicrobial therapy.. Methods: We evaluated the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the routine identification of clinical isolates of mycobacteria using 2 commercially available spectral reference libraries, and also assessed the impact of mycobacterial culture using solid medium and broth on MALDI-TOF MS–based identification.

Article Title: Azide-alkyne cycloaddition en route towards 1H-1,2,3-triazole-tethered β-lactam-ferrocene and β-lactam-ferrocenylchalcone conjugates: synthesis and in vitro anti-tubercular evaluation.
Article Snippet: A diverse range of triazoles were prepared following well established, Cu-mediated azide–alkyne cycloaddition reactions with the aim of probing the anti-tubercular structure–activity relationships (SAR) within the β-lactam–ferrocene–triazole conjugate family.. The anti-tubercular evaluation studies of the synthesized conjugates revealed that none of the scaffolds exhibited any activity that restricted mycobacterial growth even at high doses.. The introduction of various substituents onto the N-1 of the β-lactam ring, introducing monoor di-ferrocenylchalcone substituents at the C-3 position as well as introducing a spacer of varying chain length failed to produce any significant enhancement in the activity profiles.

Article Title: An attenuated Mycobacterium tuberculosis clinical strain with a defect in ESX-1 secretion induces minimal host immune responses and pathology
Article Snippet: All M.tb strains used in this study were grown at 37 °C for 3–4 weeks either on solid medium (Middlebrook 7H11) or in liquid medium (Middlebrook 7H9) supplemented with 10% (v/v) oleic acid–albumin–dextrose–catalase (OADC).

Article Title: Rifampicin and isoniazid resistance not promote fluoroquinolone resistance in Mycobacterium smegmatis
Article Snippet: Spontaneous FQ-resistant mutant colonies were selected on Middlebrook 7H11 solid medium with 10% OADC (7H11-OADC) and 1.0 μg/mL levofloxacin (2× MIC).

Article Title: Experimental Model of Tuberculosis in the Domestic Goat after Endobronchial Infection with Mycobacterium caprae
Article Snippet: The inoculum was titrated after the inoculation in duplicate by 10-fold serial dilution in Middlebrook 7H11 solid medium; accordingly, each goat received 1.5 × 10 3 CFU of M. caprae .

Article Title: The Mycobacterium tuberculosis β-oxidation genes echA5 and fadB3 are dispensable for growth in vitro and in vivo
Article Snippet: Intracellular survival and growth was assessed by lysis of the monolayers by the addition of water followed by a 30 min incubation at room temperature and enumeration of bacteria by serial dilution in PBS-Tween plating onto Middlebrook 7H11 solid medium supplemented with 0.5% glycerol and 10% OADC.

Article Title: Identification of β-Lactams Active against Mycobacterium tuberculosis by a Consortium of Pharmaceutical Companies and Academic Institutions
Article Snippet: To plate for CFU, lung homogenates were thawed, and bacilli were enumerated on Middlebrook 7H11 solid medium supplemented with 10% OADC and 0.4% activated charcoal for ∼18 days at 37 °C.

Generated:

Article Title: Comparison of MALDI-TOF MS With HPLC and Nucleic Acid Sequencing for the Identification of Mycobacterium Species in Cultures Using Solid Medium and Broth
Article Snippet: Objectives: The genus Mycobacterium contains over 150 species including pathogenic and nonpathogenic strains.. Accurate species level identification can aid in differentiating environmental contamination from true infection, and also can aid in selection of antimicrobial therapy.. Methods: We evaluated the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the routine identification of clinical isolates of mycobacteria using 2 commercially available spectral reference libraries, and also assessed the impact of mycobacterial culture using solid medium and broth on MALDI-TOF MS–based identification.

Article Title: Azide-alkyne cycloaddition en route towards 1H-1,2,3-triazole-tethered β-lactam-ferrocene and β-lactam-ferrocenylchalcone conjugates: synthesis and in vitro anti-tubercular evaluation.
Article Snippet: A diverse range of triazoles were prepared following well established, Cu-mediated azide–alkyne cycloaddition reactions with the aim of probing the anti-tubercular structure–activity relationships (SAR) within the β-lactam–ferrocene–triazole conjugate family.. The anti-tubercular evaluation studies of the synthesized conjugates revealed that none of the scaffolds exhibited any activity that restricted mycobacterial growth even at high doses.. The introduction of various substituents onto the N-1 of the β-lactam ring, introducing monoor di-ferrocenylchalcone substituents at the C-3 position as well as introducing a spacer of varying chain length failed to produce any significant enhancement in the activity profiles.

Article Title: An attenuated Mycobacterium tuberculosis clinical strain with a defect in ESX-1 secretion induces minimal host immune responses and pathology
Article Snippet: All M.tb strains used in this study were grown at 37 °C for 3–4 weeks either on solid medium (Middlebrook 7H11) or in liquid medium (Middlebrook 7H9) supplemented with 10% (v/v) oleic acid–albumin–dextrose–catalase (OADC).

Article Title: Rifampicin and isoniazid resistance not promote fluoroquinolone resistance in Mycobacterium smegmatis
Article Snippet: Spontaneous FQ-resistant mutant colonies were selected on Middlebrook 7H11 solid medium with 10% OADC (7H11-OADC) and 1.0 μg/mL levofloxacin (2× MIC).

Article Title: Experimental Model of Tuberculosis in the Domestic Goat after Endobronchial Infection with Mycobacterium caprae
Article Snippet: The inoculum was titrated after the inoculation in duplicate by 10-fold serial dilution in Middlebrook 7H11 solid medium; accordingly, each goat received 1.5 × 10 3 CFU of M. caprae .

Article Title: The Mycobacterium tuberculosis β-oxidation genes echA5 and fadB3 are dispensable for growth in vitro and in vivo
Article Snippet: Intracellular survival and growth was assessed by lysis of the monolayers by the addition of water followed by a 30 min incubation at room temperature and enumeration of bacteria by serial dilution in PBS-Tween plating onto Middlebrook 7H11 solid medium supplemented with 0.5% glycerol and 10% OADC.

Article Title: Identification of β-Lactams Active against Mycobacterium tuberculosis by a Consortium of Pharmaceutical Companies and Academic Institutions
Article Snippet: To plate for CFU, lung homogenates were thawed, and bacilli were enumerated on Middlebrook 7H11 solid medium supplemented with 10% OADC and 0.4% activated charcoal for ∼18 days at 37 °C.

Lysis:

Article Title: Comparison of MALDI-TOF MS With HPLC and Nucleic Acid Sequencing for the Identification of Mycobacterium Species in Cultures Using Solid Medium and Broth
Article Snippet: Objectives: The genus Mycobacterium contains over 150 species including pathogenic and nonpathogenic strains.. Accurate species level identification can aid in differentiating environmental contamination from true infection, and also can aid in selection of antimicrobial therapy.. Methods: We evaluated the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the routine identification of clinical isolates of mycobacteria using 2 commercially available spectral reference libraries, and also assessed the impact of mycobacterial culture using solid medium and broth on MALDI-TOF MS–based identification.

Article Title: Azide-alkyne cycloaddition en route towards 1H-1,2,3-triazole-tethered β-lactam-ferrocene and β-lactam-ferrocenylchalcone conjugates: synthesis and in vitro anti-tubercular evaluation.
Article Snippet: A diverse range of triazoles were prepared following well established, Cu-mediated azide–alkyne cycloaddition reactions with the aim of probing the anti-tubercular structure–activity relationships (SAR) within the β-lactam–ferrocene–triazole conjugate family.. The anti-tubercular evaluation studies of the synthesized conjugates revealed that none of the scaffolds exhibited any activity that restricted mycobacterial growth even at high doses.. The introduction of various substituents onto the N-1 of the β-lactam ring, introducing monoor di-ferrocenylchalcone substituents at the C-3 position as well as introducing a spacer of varying chain length failed to produce any significant enhancement in the activity profiles.

Article Title: An attenuated Mycobacterium tuberculosis clinical strain with a defect in ESX-1 secretion induces minimal host immune responses and pathology
Article Snippet: All M.tb strains used in this study were grown at 37 °C for 3–4 weeks either on solid medium (Middlebrook 7H11) or in liquid medium (Middlebrook 7H9) supplemented with 10% (v/v) oleic acid–albumin–dextrose–catalase (OADC).

Article Title: Rifampicin and isoniazid resistance not promote fluoroquinolone resistance in Mycobacterium smegmatis
Article Snippet: Spontaneous FQ-resistant mutant colonies were selected on Middlebrook 7H11 solid medium with 10% OADC (7H11-OADC) and 1.0 μg/mL levofloxacin (2× MIC).

Article Title: Experimental Model of Tuberculosis in the Domestic Goat after Endobronchial Infection with Mycobacterium caprae
Article Snippet: The inoculum was titrated after the inoculation in duplicate by 10-fold serial dilution in Middlebrook 7H11 solid medium; accordingly, each goat received 1.5 × 10 3 CFU of M. caprae .

Article Title: The Mycobacterium tuberculosis β-oxidation genes echA5 and fadB3 are dispensable for growth in vitro and in vivo
Article Snippet: Intracellular survival and growth was assessed by lysis of the monolayers by the addition of water followed by a 30 min incubation at room temperature and enumeration of bacteria by serial dilution in PBS-Tween plating onto Middlebrook 7H11 solid medium supplemented with 0.5% glycerol and 10% OADC.

Article Title: Identification of β-Lactams Active against Mycobacterium tuberculosis by a Consortium of Pharmaceutical Companies and Academic Institutions
Article Snippet: To plate for CFU, lung homogenates were thawed, and bacilli were enumerated on Middlebrook 7H11 solid medium supplemented with 10% OADC and 0.4% activated charcoal for ∼18 days at 37 °C.

Incubation:

Article Title: Comparison of MALDI-TOF MS With HPLC and Nucleic Acid Sequencing for the Identification of Mycobacterium Species in Cultures Using Solid Medium and Broth
Article Snippet: Objectives: The genus Mycobacterium contains over 150 species including pathogenic and nonpathogenic strains.. Accurate species level identification can aid in differentiating environmental contamination from true infection, and also can aid in selection of antimicrobial therapy.. Methods: We evaluated the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the routine identification of clinical isolates of mycobacteria using 2 commercially available spectral reference libraries, and also assessed the impact of mycobacterial culture using solid medium and broth on MALDI-TOF MS–based identification.

Article Title: Azide-alkyne cycloaddition en route towards 1H-1,2,3-triazole-tethered β-lactam-ferrocene and β-lactam-ferrocenylchalcone conjugates: synthesis and in vitro anti-tubercular evaluation.
Article Snippet: A diverse range of triazoles were prepared following well established, Cu-mediated azide–alkyne cycloaddition reactions with the aim of probing the anti-tubercular structure–activity relationships (SAR) within the β-lactam–ferrocene–triazole conjugate family.. The anti-tubercular evaluation studies of the synthesized conjugates revealed that none of the scaffolds exhibited any activity that restricted mycobacterial growth even at high doses.. The introduction of various substituents onto the N-1 of the β-lactam ring, introducing monoor di-ferrocenylchalcone substituents at the C-3 position as well as introducing a spacer of varying chain length failed to produce any significant enhancement in the activity profiles.

Article Title: An attenuated Mycobacterium tuberculosis clinical strain with a defect in ESX-1 secretion induces minimal host immune responses and pathology
Article Snippet: All M.tb strains used in this study were grown at 37 °C for 3–4 weeks either on solid medium (Middlebrook 7H11) or in liquid medium (Middlebrook 7H9) supplemented with 10% (v/v) oleic acid–albumin–dextrose–catalase (OADC).

Article Title: Rifampicin and isoniazid resistance not promote fluoroquinolone resistance in Mycobacterium smegmatis
Article Snippet: Spontaneous FQ-resistant mutant colonies were selected on Middlebrook 7H11 solid medium with 10% OADC (7H11-OADC) and 1.0 μg/mL levofloxacin (2× MIC).

Article Title: Experimental Model of Tuberculosis in the Domestic Goat after Endobronchial Infection with Mycobacterium caprae
Article Snippet: The inoculum was titrated after the inoculation in duplicate by 10-fold serial dilution in Middlebrook 7H11 solid medium; accordingly, each goat received 1.5 × 10 3 CFU of M. caprae .

Article Title: The Mycobacterium tuberculosis β-oxidation genes echA5 and fadB3 are dispensable for growth in vitro and in vivo
Article Snippet: Intracellular survival and growth was assessed by lysis of the monolayers by the addition of water followed by a 30 min incubation at room temperature and enumeration of bacteria by serial dilution in PBS-Tween plating onto Middlebrook 7H11 solid medium supplemented with 0.5% glycerol and 10% OADC.

Article Title: Identification of β-Lactams Active against Mycobacterium tuberculosis by a Consortium of Pharmaceutical Companies and Academic Institutions
Article Snippet: To plate for CFU, lung homogenates were thawed, and bacilli were enumerated on Middlebrook 7H11 solid medium supplemented with 10% OADC and 0.4% activated charcoal for ∼18 days at 37 °C.

Serial Dilution:

Article Title: Comparison of MALDI-TOF MS With HPLC and Nucleic Acid Sequencing for the Identification of Mycobacterium Species in Cultures Using Solid Medium and Broth
Article Snippet: Objectives: The genus Mycobacterium contains over 150 species including pathogenic and nonpathogenic strains.. Accurate species level identification can aid in differentiating environmental contamination from true infection, and also can aid in selection of antimicrobial therapy.. Methods: We evaluated the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the routine identification of clinical isolates of mycobacteria using 2 commercially available spectral reference libraries, and also assessed the impact of mycobacterial culture using solid medium and broth on MALDI-TOF MS–based identification.

Article Title: Azide-alkyne cycloaddition en route towards 1H-1,2,3-triazole-tethered β-lactam-ferrocene and β-lactam-ferrocenylchalcone conjugates: synthesis and in vitro anti-tubercular evaluation.
Article Snippet: A diverse range of triazoles were prepared following well established, Cu-mediated azide–alkyne cycloaddition reactions with the aim of probing the anti-tubercular structure–activity relationships (SAR) within the β-lactam–ferrocene–triazole conjugate family.. The anti-tubercular evaluation studies of the synthesized conjugates revealed that none of the scaffolds exhibited any activity that restricted mycobacterial growth even at high doses.. The introduction of various substituents onto the N-1 of the β-lactam ring, introducing monoor di-ferrocenylchalcone substituents at the C-3 position as well as introducing a spacer of varying chain length failed to produce any significant enhancement in the activity profiles.

Article Title: An attenuated Mycobacterium tuberculosis clinical strain with a defect in ESX-1 secretion induces minimal host immune responses and pathology
Article Snippet: All M.tb strains used in this study were grown at 37 °C for 3–4 weeks either on solid medium (Middlebrook 7H11) or in liquid medium (Middlebrook 7H9) supplemented with 10% (v/v) oleic acid–albumin–dextrose–catalase (OADC).

Article Title: Rifampicin and isoniazid resistance not promote fluoroquinolone resistance in Mycobacterium smegmatis
Article Snippet: Spontaneous FQ-resistant mutant colonies were selected on Middlebrook 7H11 solid medium with 10% OADC (7H11-OADC) and 1.0 μg/mL levofloxacin (2× MIC).

Article Title: Experimental Model of Tuberculosis in the Domestic Goat after Endobronchial Infection with Mycobacterium caprae
Article Snippet: The inoculum was titrated after the inoculation in duplicate by 10-fold serial dilution in Middlebrook 7H11 solid medium; accordingly, each goat received 1.5 × 10 3 CFU of M. caprae .

Article Title: The Mycobacterium tuberculosis β-oxidation genes echA5 and fadB3 are dispensable for growth in vitro and in vivo
Article Snippet: Intracellular survival and growth was assessed by lysis of the monolayers by the addition of water followed by a 30 min incubation at room temperature and enumeration of bacteria by serial dilution in PBS-Tween plating onto Middlebrook 7H11 solid medium supplemented with 0.5% glycerol and 10% OADC.

Article Title: Identification of β-Lactams Active against Mycobacterium tuberculosis by a Consortium of Pharmaceutical Companies and Academic Institutions
Article Snippet: To plate for CFU, lung homogenates were thawed, and bacilli were enumerated on Middlebrook 7H11 solid medium supplemented with 10% OADC and 0.4% activated charcoal for ∼18 days at 37 °C.



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MiddleBrook Pharmaceuticals modified middlebrook 7h11 solid medium
Using CRISPRi/dCas9 to inhibit wag31 expression in M. bovis and M. tuberculosis . (A) Schematic showing the target regions of two sgRNAs designed to target and inhibit wag31 expression. The numbers show the nucleotide position of the sgRNA relative to the annotated start site. (B) CRISPRi strains were cultured in 10 ml of supplemented 7H9 medium to exponential phase and diluted to 2 × 10 7 CFU/ml. A 10-fold serial dilution to 10 −4 was performed and 20 μl of each dilution was spotted onto <t>7H11</t> agar without aTc and with 200 ng/ml aTc to induce CRISPRi/dCas9 and the sgRNA in those strains that carried the guide. Two biological replicates were carried out.
Modified Middlebrook 7h11 Solid Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/solid+medium+middlebrook+7h11/middlebrook+7h9+broth/pmc08739905-86-8-7
Average 90 stars, based on 1 article reviews
modified middlebrook 7h11 solid medium - by Bioz Stars, 2026-09
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MiddleBrook Pharmaceuticals solid modified 7h11 middlebrook medium plates
Using CRISPRi/dCas9 to inhibit wag31 expression in M. bovis and M. tuberculosis . (A) Schematic showing the target regions of two sgRNAs designed to target and inhibit wag31 expression. The numbers show the nucleotide position of the sgRNA relative to the annotated start site. (B) CRISPRi strains were cultured in 10 ml of supplemented 7H9 medium to exponential phase and diluted to 2 × 10 7 CFU/ml. A 10-fold serial dilution to 10 −4 was performed and 20 μl of each dilution was spotted onto <t>7H11</t> agar without aTc and with 200 ng/ml aTc to induce CRISPRi/dCas9 and the sgRNA in those strains that carried the guide. Two biological replicates were carried out.
Solid Modified 7h11 Middlebrook Medium Plates, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/solid+medium+middlebrook+7h11/middlebrook+7h11+medium/pm31733947-83-5-8
Average 90 stars, based on 1 article reviews
solid modified 7h11 middlebrook medium plates - by Bioz Stars, 2026-09
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Difco middlebrook 7h11 solid medium
Using CRISPRi/dCas9 to inhibit wag31 expression in M. bovis and M. tuberculosis . (A) Schematic showing the target regions of two sgRNAs designed to target and inhibit wag31 expression. The numbers show the nucleotide position of the sgRNA relative to the annotated start site. (B) CRISPRi strains were cultured in 10 ml of supplemented 7H9 medium to exponential phase and diluted to 2 × 10 7 CFU/ml. A 10-fold serial dilution to 10 −4 was performed and 20 μl of each dilution was spotted onto <t>7H11</t> agar without aTc and with 200 ng/ml aTc to induce CRISPRi/dCas9 and the sgRNA in those strains that carried the guide. Two biological replicates were carried out.
Middlebrook 7h11 Solid Medium, supplied by Difco, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/solid+medium+middlebrook+7h11/middlebrook+7h10+agar/pmc08208694-52-34-37
Average 90 stars, based on 1 article reviews
middlebrook 7h11 solid medium - by Bioz Stars, 2026-09
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Difco middlebrook 7h11 solid culture medium
Using CRISPRi/dCas9 to inhibit wag31 expression in M. bovis and M. tuberculosis . (A) Schematic showing the target regions of two sgRNAs designed to target and inhibit wag31 expression. The numbers show the nucleotide position of the sgRNA relative to the annotated start site. (B) CRISPRi strains were cultured in 10 ml of supplemented 7H9 medium to exponential phase and diluted to 2 × 10 7 CFU/ml. A 10-fold serial dilution to 10 −4 was performed and 20 μl of each dilution was spotted onto <t>7H11</t> agar without aTc and with 200 ng/ml aTc to induce CRISPRi/dCas9 and the sgRNA in those strains that carried the guide. Two biological replicates were carried out.
Middlebrook 7h11 Solid Culture Medium, supplied by Difco, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/solid+medium+middlebrook+7h11/middlebrook+7h10+agar/pm31430695-32-32-33
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middlebrook 7h11 solid culture medium - by Bioz Stars, 2026-09
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Becton Dickinson solid middlebrook 7h11 medium
Using CRISPRi/dCas9 to inhibit wag31 expression in M. bovis and M. tuberculosis . (A) Schematic showing the target regions of two sgRNAs designed to target and inhibit wag31 expression. The numbers show the nucleotide position of the sgRNA relative to the annotated start site. (B) CRISPRi strains were cultured in 10 ml of supplemented 7H9 medium to exponential phase and diluted to 2 × 10 7 CFU/ml. A 10-fold serial dilution to 10 −4 was performed and 20 μl of each dilution was spotted onto <t>7H11</t> agar without aTc and with 200 ng/ml aTc to induce CRISPRi/dCas9 and the sgRNA in those strains that carried the guide. Two biological replicates were carried out.
Solid Middlebrook 7h11 Medium, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/solid+medium+middlebrook+7h11/middlebrook+7h10+agar/pm30029916-55-24-26
Average 90 stars, based on 1 article reviews
solid middlebrook 7h11 medium - by Bioz Stars, 2026-09
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MiddleBrook Pharmaceuticals solid agar medium middlebrook 7h11
Using CRISPRi/dCas9 to inhibit wag31 expression in M. bovis and M. tuberculosis . (A) Schematic showing the target regions of two sgRNAs designed to target and inhibit wag31 expression. The numbers show the nucleotide position of the sgRNA relative to the annotated start site. (B) CRISPRi strains were cultured in 10 ml of supplemented 7H9 medium to exponential phase and diluted to 2 × 10 7 CFU/ml. A 10-fold serial dilution to 10 −4 was performed and 20 μl of each dilution was spotted onto <t>7H11</t> agar without aTc and with 200 ng/ml aTc to induce CRISPRi/dCas9 and the sgRNA in those strains that carried the guide. Two biological replicates were carried out.
Solid Agar Medium Middlebrook 7h11, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/solid+medium+middlebrook+7h11/middlebrook+7h11+medium/pmc06433132-58-10-12
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solid agar medium middlebrook 7h11 - by Bioz Stars, 2026-09
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Using CRISPRi/dCas9 to inhibit wag31 expression in M. bovis and M. tuberculosis . (A) Schematic showing the target regions of two sgRNAs designed to target and inhibit wag31 expression. The numbers show the nucleotide position of the sgRNA relative to the annotated start site. (B) CRISPRi strains were cultured in 10 ml of supplemented 7H9 medium to exponential phase and diluted to 2 × 10 7 CFU/ml. A 10-fold serial dilution to 10 −4 was performed and 20 μl of each dilution was spotted onto 7H11 agar without aTc and with 200 ng/ml aTc to induce CRISPRi/dCas9 and the sgRNA in those strains that carried the guide. Two biological replicates were carried out.

Journal: Frontiers in Veterinary Science

Article Title: Probing Differences in Gene Essentiality Between the Human and Animal Adapted Lineages of the Mycobacterium tuberculosis Complex Using TnSeq

doi: 10.3389/fvets.2021.760717

Figure Lengend Snippet: Using CRISPRi/dCas9 to inhibit wag31 expression in M. bovis and M. tuberculosis . (A) Schematic showing the target regions of two sgRNAs designed to target and inhibit wag31 expression. The numbers show the nucleotide position of the sgRNA relative to the annotated start site. (B) CRISPRi strains were cultured in 10 ml of supplemented 7H9 medium to exponential phase and diluted to 2 × 10 7 CFU/ml. A 10-fold serial dilution to 10 −4 was performed and 20 μl of each dilution was spotted onto 7H11 agar without aTc and with 200 ng/ml aTc to induce CRISPRi/dCas9 and the sgRNA in those strains that carried the guide. Two biological replicates were carried out.

Article Snippet: M. bovis AF2122/97 was maintained on modified Middlebrook 7H11 solid medium containing 0.5% lysed defibrinated sheep blood, 10% heat inactivated foetal bovine serum and 10% oleic acid-albumin-dextrose-catalase (OADC) ( ).

Techniques: Expressing, Cell Culture, Serial Dilution

Using CRISPRi/dCas9 to inhibit Rv2182c/Mb2204c expression in M. bovis and M. tuberculosis . (A) Schematic showing the target regions of two sgRNAs designed to target and inhibit Rv2182c/Mb2204c expression. The numbers show the nucleotide position of the sgRNA relative to the annotated start site (B) dCas9 expression and Rv2182c/Mb2204c expression were measured by RT-qPCR as described in the methods section. Expression levels were measured in the strain expressing sgRNA agpat1. Gene expression was analysed using the 2 −Δ ΔCT method, normalised against sigA . Results represent two biological repeats with two technical repeats each. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 or not significant (ns), analysed using a 2-way ANOVA test. Black bars represent aTc treated cultures and grey bars untreated. (C) CRISPRi strains were cultured in 10 ml of supplemented 7H9 medium to exponential phase and diluted to 2 × 10 7 CFU/ml. A 10-fold serial dilution to 10 −4 was performed and 20 μl of each dilution was spotted onto 7H11 agar without aTc and with 200 ng/ml aTc to induce CRISPRi/dCas9 and the sgRNA in those strains that carried the guide. Two biological replicates were carried out.

Journal: Frontiers in Veterinary Science

Article Title: Probing Differences in Gene Essentiality Between the Human and Animal Adapted Lineages of the Mycobacterium tuberculosis Complex Using TnSeq

doi: 10.3389/fvets.2021.760717

Figure Lengend Snippet: Using CRISPRi/dCas9 to inhibit Rv2182c/Mb2204c expression in M. bovis and M. tuberculosis . (A) Schematic showing the target regions of two sgRNAs designed to target and inhibit Rv2182c/Mb2204c expression. The numbers show the nucleotide position of the sgRNA relative to the annotated start site (B) dCas9 expression and Rv2182c/Mb2204c expression were measured by RT-qPCR as described in the methods section. Expression levels were measured in the strain expressing sgRNA agpat1. Gene expression was analysed using the 2 −Δ ΔCT method, normalised against sigA . Results represent two biological repeats with two technical repeats each. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 or not significant (ns), analysed using a 2-way ANOVA test. Black bars represent aTc treated cultures and grey bars untreated. (C) CRISPRi strains were cultured in 10 ml of supplemented 7H9 medium to exponential phase and diluted to 2 × 10 7 CFU/ml. A 10-fold serial dilution to 10 −4 was performed and 20 μl of each dilution was spotted onto 7H11 agar without aTc and with 200 ng/ml aTc to induce CRISPRi/dCas9 and the sgRNA in those strains that carried the guide. Two biological replicates were carried out.

Article Snippet: M. bovis AF2122/97 was maintained on modified Middlebrook 7H11 solid medium containing 0.5% lysed defibrinated sheep blood, 10% heat inactivated foetal bovine serum and 10% oleic acid-albumin-dextrose-catalase (OADC) ( ).

Techniques: Expressing, Quantitative RT-PCR, Cell Culture, Serial Dilution