solid medium middlebrook 7h11 (MiddleBrook Pharmaceuticals)
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Solid Medium Middlebrook 7h11, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/solid+medium+middlebrook+7h11/middlebrook+7h9+broth/pmc11694965-45-7-7
Average 90 stars, based on 1 article reviews
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Activity Assay:Article Title: Comparison of MALDI-TOF MS With HPLC and Nucleic Acid Sequencing for the Identification of Mycobacterium Species in Cultures Using Solid Medium and Broth Article Snippet: Objectives: The genus Mycobacterium contains over 150 species including pathogenic and nonpathogenic strains.. Accurate species level identification can aid in differentiating environmental contamination from true infection, and also can aid in selection of antimicrobial therapy.. Methods: We evaluated the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the routine identification of clinical isolates of mycobacteria using 2 commercially available spectral reference libraries, and also assessed the impact of mycobacterial culture using solid medium and broth on MALDI-TOF MS–based identification. Article Title: Azide-alkyne cycloaddition en route towards 1H-1,2,3-triazole-tethered β-lactam-ferrocene and β-lactam-ferrocenylchalcone conjugates: synthesis and in vitro anti-tubercular evaluation. Article Snippet: A diverse range of triazoles were prepared following well established, Cu-mediated azide–alkyne cycloaddition reactions with the aim of probing the anti-tubercular structure–activity relationships (SAR) within the β-lactam–ferrocene–triazole conjugate family.. The anti-tubercular evaluation studies of the synthesized conjugates revealed that none of the scaffolds exhibited any activity that restricted mycobacterial growth even at high doses.. The introduction of various substituents onto the N-1 of the β-lactam ring, introducing monoor di-ferrocenylchalcone substituents at the C-3 position as well as introducing a spacer of varying chain length failed to produce any significant enhancement in the activity profiles. Article Title: An attenuated Mycobacterium tuberculosis clinical strain with a defect in ESX-1 secretion induces minimal host immune responses and pathology Article Snippet: All M.tb strains used in this study were grown at 37 °C for 3–4 weeks either on solid Article Title: Rifampicin and isoniazid resistance not promote fluoroquinolone resistance in Mycobacterium smegmatis Article Snippet: Spontaneous FQ-resistant mutant colonies were selected on Article Title: Experimental Model of Tuberculosis in the Domestic Goat after Endobronchial Infection with Mycobacterium caprae Article Snippet: The inoculum was titrated after the inoculation in duplicate by 10-fold serial dilution in Article Title: The Mycobacterium tuberculosis β-oxidation genes echA5 and fadB3 are dispensable for growth in vitro and in vivo Article Snippet: Intracellular survival and growth was assessed by lysis of the monolayers by the addition of water followed by a 30 min incubation at Article Title: Identification of β-Lactams Active against Mycobacterium tuberculosis by a Consortium of Pharmaceutical Companies and Academic Institutions Article Snippet: To plate for CFU, lung homogenates were thawed, and bacilli were enumerated on Knock-Out:Article Title: Comparison of MALDI-TOF MS With HPLC and Nucleic Acid Sequencing for the Identification of Mycobacterium Species in Cultures Using Solid Medium and Broth Article Snippet: Objectives: The genus Mycobacterium contains over 150 species including pathogenic and nonpathogenic strains.. Accurate species level identification can aid in differentiating environmental contamination from true infection, and also can aid in selection of antimicrobial therapy.. Methods: We evaluated the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the routine identification of clinical isolates of mycobacteria using 2 commercially available spectral reference libraries, and also assessed the impact of mycobacterial culture using solid medium and broth on MALDI-TOF MS–based identification. Article Title: Azide-alkyne cycloaddition en route towards 1H-1,2,3-triazole-tethered β-lactam-ferrocene and β-lactam-ferrocenylchalcone conjugates: synthesis and in vitro anti-tubercular evaluation. Article Snippet: A diverse range of triazoles were prepared following well established, Cu-mediated azide–alkyne cycloaddition reactions with the aim of probing the anti-tubercular structure–activity relationships (SAR) within the β-lactam–ferrocene–triazole conjugate family.. The anti-tubercular evaluation studies of the synthesized conjugates revealed that none of the scaffolds exhibited any activity that restricted mycobacterial growth even at high doses.. The introduction of various substituents onto the N-1 of the β-lactam ring, introducing monoor di-ferrocenylchalcone substituents at the C-3 position as well as introducing a spacer of varying chain length failed to produce any significant enhancement in the activity profiles. Article Title: An attenuated Mycobacterium tuberculosis clinical strain with a defect in ESX-1 secretion induces minimal host immune responses and pathology Article Snippet: All M.tb strains used in this study were grown at 37 °C for 3–4 weeks either on solid Article Title: Rifampicin and isoniazid resistance not promote fluoroquinolone resistance in Mycobacterium smegmatis Article Snippet: Spontaneous FQ-resistant mutant colonies were selected on Article Title: Experimental Model of Tuberculosis in the Domestic Goat after Endobronchial Infection with Mycobacterium caprae Article Snippet: The inoculum was titrated after the inoculation in duplicate by 10-fold serial dilution in Article Title: The Mycobacterium tuberculosis β-oxidation genes echA5 and fadB3 are dispensable for growth in vitro and in vivo Article Snippet: Intracellular survival and growth was assessed by lysis of the monolayers by the addition of water followed by a 30 min incubation at Article Title: Identification of β-Lactams Active against Mycobacterium tuberculosis by a Consortium of Pharmaceutical Companies and Academic Institutions Article Snippet: To plate for CFU, lung homogenates were thawed, and bacilli were enumerated on Infection:Article Title: Comparison of MALDI-TOF MS With HPLC and Nucleic Acid Sequencing for the Identification of Mycobacterium Species in Cultures Using Solid Medium and Broth Article Snippet: Objectives: The genus Mycobacterium contains over 150 species including pathogenic and nonpathogenic strains.. Accurate species level identification can aid in differentiating environmental contamination from true infection, and also can aid in selection of antimicrobial therapy.. Methods: We evaluated the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the routine identification of clinical isolates of mycobacteria using 2 commercially available spectral reference libraries, and also assessed the impact of mycobacterial culture using solid medium and broth on MALDI-TOF MS–based identification. Article Title: Azide-alkyne cycloaddition en route towards 1H-1,2,3-triazole-tethered β-lactam-ferrocene and β-lactam-ferrocenylchalcone conjugates: synthesis and in vitro anti-tubercular evaluation. Article Snippet: A diverse range of triazoles were prepared following well established, Cu-mediated azide–alkyne cycloaddition reactions with the aim of probing the anti-tubercular structure–activity relationships (SAR) within the β-lactam–ferrocene–triazole conjugate family.. The anti-tubercular evaluation studies of the synthesized conjugates revealed that none of the scaffolds exhibited any activity that restricted mycobacterial growth even at high doses.. The introduction of various substituents onto the N-1 of the β-lactam ring, introducing monoor di-ferrocenylchalcone substituents at the C-3 position as well as introducing a spacer of varying chain length failed to produce any significant enhancement in the activity profiles. Article Title: An attenuated Mycobacterium tuberculosis clinical strain with a defect in ESX-1 secretion induces minimal host immune responses and pathology Article Snippet: All M.tb strains used in this study were grown at 37 °C for 3–4 weeks either on solid Article Title: Rifampicin and isoniazid resistance not promote fluoroquinolone resistance in Mycobacterium smegmatis Article Snippet: Spontaneous FQ-resistant mutant colonies were selected on Article Title: Experimental Model of Tuberculosis in the Domestic Goat after Endobronchial Infection with Mycobacterium caprae Article Snippet: The inoculum was titrated after the inoculation in duplicate by 10-fold serial dilution in Article Title: The Mycobacterium tuberculosis β-oxidation genes echA5 and fadB3 are dispensable for growth in vitro and in vivo Article Snippet: Intracellular survival and growth was assessed by lysis of the monolayers by the addition of water followed by a 30 min incubation at Article Title: Identification of β-Lactams Active against Mycobacterium tuberculosis by a Consortium of Pharmaceutical Companies and Academic Institutions Article Snippet: To plate for CFU, lung homogenates were thawed, and bacilli were enumerated on Software:Article Title: Comparison of MALDI-TOF MS With HPLC and Nucleic Acid Sequencing for the Identification of Mycobacterium Species in Cultures Using Solid Medium and Broth Article Snippet: Objectives: The genus Mycobacterium contains over 150 species including pathogenic and nonpathogenic strains.. Accurate species level identification can aid in differentiating environmental contamination from true infection, and also can aid in selection of antimicrobial therapy.. Methods: We evaluated the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the routine identification of clinical isolates of mycobacteria using 2 commercially available spectral reference libraries, and also assessed the impact of mycobacterial culture using solid medium and broth on MALDI-TOF MS–based identification. Article Title: Azide-alkyne cycloaddition en route towards 1H-1,2,3-triazole-tethered β-lactam-ferrocene and β-lactam-ferrocenylchalcone conjugates: synthesis and in vitro anti-tubercular evaluation. Article Snippet: A diverse range of triazoles were prepared following well established, Cu-mediated azide–alkyne cycloaddition reactions with the aim of probing the anti-tubercular structure–activity relationships (SAR) within the β-lactam–ferrocene–triazole conjugate family.. The anti-tubercular evaluation studies of the synthesized conjugates revealed that none of the scaffolds exhibited any activity that restricted mycobacterial growth even at high doses.. The introduction of various substituents onto the N-1 of the β-lactam ring, introducing monoor di-ferrocenylchalcone substituents at the C-3 position as well as introducing a spacer of varying chain length failed to produce any significant enhancement in the activity profiles. Article Title: An attenuated Mycobacterium tuberculosis clinical strain with a defect in ESX-1 secretion induces minimal host immune responses and pathology Article Snippet: All M.tb strains used in this study were grown at 37 °C for 3–4 weeks either on solid Article Title: Rifampicin and isoniazid resistance not promote fluoroquinolone resistance in Mycobacterium smegmatis Article Snippet: Spontaneous FQ-resistant mutant colonies were selected on Article Title: Experimental Model of Tuberculosis in the Domestic Goat after Endobronchial Infection with Mycobacterium caprae Article Snippet: The inoculum was titrated after the inoculation in duplicate by 10-fold serial dilution in Article Title: The Mycobacterium tuberculosis β-oxidation genes echA5 and fadB3 are dispensable for growth in vitro and in vivo Article Snippet: Intracellular survival and growth was assessed by lysis of the monolayers by the addition of water followed by a 30 min incubation at Article Title: Identification of β-Lactams Active against Mycobacterium tuberculosis by a Consortium of Pharmaceutical Companies and Academic Institutions Article Snippet: To plate for CFU, lung homogenates were thawed, and bacilli were enumerated on Cell Culture:Article Title: Comparison of MALDI-TOF MS With HPLC and Nucleic Acid Sequencing for the Identification of Mycobacterium Species in Cultures Using Solid Medium and Broth Article Snippet: Objectives: The genus Mycobacterium contains over 150 species including pathogenic and nonpathogenic strains.. Accurate species level identification can aid in differentiating environmental contamination from true infection, and also can aid in selection of antimicrobial therapy.. Methods: We evaluated the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the routine identification of clinical isolates of mycobacteria using 2 commercially available spectral reference libraries, and also assessed the impact of mycobacterial culture using solid medium and broth on MALDI-TOF MS–based identification. Article Title: Azide-alkyne cycloaddition en route towards 1H-1,2,3-triazole-tethered β-lactam-ferrocene and β-lactam-ferrocenylchalcone conjugates: synthesis and in vitro anti-tubercular evaluation. Article Snippet: A diverse range of triazoles were prepared following well established, Cu-mediated azide–alkyne cycloaddition reactions with the aim of probing the anti-tubercular structure–activity relationships (SAR) within the β-lactam–ferrocene–triazole conjugate family.. The anti-tubercular evaluation studies of the synthesized conjugates revealed that none of the scaffolds exhibited any activity that restricted mycobacterial growth even at high doses.. The introduction of various substituents onto the N-1 of the β-lactam ring, introducing monoor di-ferrocenylchalcone substituents at the C-3 position as well as introducing a spacer of varying chain length failed to produce any significant enhancement in the activity profiles. Article Title: An attenuated Mycobacterium tuberculosis clinical strain with a defect in ESX-1 secretion induces minimal host immune responses and pathology Article Snippet: All M.tb strains used in this study were grown at 37 °C for 3–4 weeks either on solid Article Title: Rifampicin and isoniazid resistance not promote fluoroquinolone resistance in Mycobacterium smegmatis Article Snippet: Spontaneous FQ-resistant mutant colonies were selected on Article Title: Experimental Model of Tuberculosis in the Domestic Goat after Endobronchial Infection with Mycobacterium caprae Article Snippet: The inoculum was titrated after the inoculation in duplicate by 10-fold serial dilution in Article Title: The Mycobacterium tuberculosis β-oxidation genes echA5 and fadB3 are dispensable for growth in vitro and in vivo Article Snippet: Intracellular survival and growth was assessed by lysis of the monolayers by the addition of water followed by a 30 min incubation at Article Title: Identification of β-Lactams Active against Mycobacterium tuberculosis by a Consortium of Pharmaceutical Companies and Academic Institutions Article Snippet: To plate for CFU, lung homogenates were thawed, and bacilli were enumerated on Generated:Article Title: Comparison of MALDI-TOF MS With HPLC and Nucleic Acid Sequencing for the Identification of Mycobacterium Species in Cultures Using Solid Medium and Broth Article Snippet: Objectives: The genus Mycobacterium contains over 150 species including pathogenic and nonpathogenic strains.. Accurate species level identification can aid in differentiating environmental contamination from true infection, and also can aid in selection of antimicrobial therapy.. Methods: We evaluated the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the routine identification of clinical isolates of mycobacteria using 2 commercially available spectral reference libraries, and also assessed the impact of mycobacterial culture using solid medium and broth on MALDI-TOF MS–based identification. Article Title: Azide-alkyne cycloaddition en route towards 1H-1,2,3-triazole-tethered β-lactam-ferrocene and β-lactam-ferrocenylchalcone conjugates: synthesis and in vitro anti-tubercular evaluation. Article Snippet: A diverse range of triazoles were prepared following well established, Cu-mediated azide–alkyne cycloaddition reactions with the aim of probing the anti-tubercular structure–activity relationships (SAR) within the β-lactam–ferrocene–triazole conjugate family.. The anti-tubercular evaluation studies of the synthesized conjugates revealed that none of the scaffolds exhibited any activity that restricted mycobacterial growth even at high doses.. The introduction of various substituents onto the N-1 of the β-lactam ring, introducing monoor di-ferrocenylchalcone substituents at the C-3 position as well as introducing a spacer of varying chain length failed to produce any significant enhancement in the activity profiles. Article Title: An attenuated Mycobacterium tuberculosis clinical strain with a defect in ESX-1 secretion induces minimal host immune responses and pathology Article Snippet: All M.tb strains used in this study were grown at 37 °C for 3–4 weeks either on solid Article Title: Rifampicin and isoniazid resistance not promote fluoroquinolone resistance in Mycobacterium smegmatis Article Snippet: Spontaneous FQ-resistant mutant colonies were selected on Article Title: Experimental Model of Tuberculosis in the Domestic Goat after Endobronchial Infection with Mycobacterium caprae Article Snippet: The inoculum was titrated after the inoculation in duplicate by 10-fold serial dilution in Article Title: The Mycobacterium tuberculosis β-oxidation genes echA5 and fadB3 are dispensable for growth in vitro and in vivo Article Snippet: Intracellular survival and growth was assessed by lysis of the monolayers by the addition of water followed by a 30 min incubation at Article Title: Identification of β-Lactams Active against Mycobacterium tuberculosis by a Consortium of Pharmaceutical Companies and Academic Institutions Article Snippet: To plate for CFU, lung homogenates were thawed, and bacilli were enumerated on Lysis:Article Title: Comparison of MALDI-TOF MS With HPLC and Nucleic Acid Sequencing for the Identification of Mycobacterium Species in Cultures Using Solid Medium and Broth Article Snippet: Objectives: The genus Mycobacterium contains over 150 species including pathogenic and nonpathogenic strains.. Accurate species level identification can aid in differentiating environmental contamination from true infection, and also can aid in selection of antimicrobial therapy.. Methods: We evaluated the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the routine identification of clinical isolates of mycobacteria using 2 commercially available spectral reference libraries, and also assessed the impact of mycobacterial culture using solid medium and broth on MALDI-TOF MS–based identification. Article Title: Azide-alkyne cycloaddition en route towards 1H-1,2,3-triazole-tethered β-lactam-ferrocene and β-lactam-ferrocenylchalcone conjugates: synthesis and in vitro anti-tubercular evaluation. Article Snippet: A diverse range of triazoles were prepared following well established, Cu-mediated azide–alkyne cycloaddition reactions with the aim of probing the anti-tubercular structure–activity relationships (SAR) within the β-lactam–ferrocene–triazole conjugate family.. The anti-tubercular evaluation studies of the synthesized conjugates revealed that none of the scaffolds exhibited any activity that restricted mycobacterial growth even at high doses.. The introduction of various substituents onto the N-1 of the β-lactam ring, introducing monoor di-ferrocenylchalcone substituents at the C-3 position as well as introducing a spacer of varying chain length failed to produce any significant enhancement in the activity profiles. Article Title: An attenuated Mycobacterium tuberculosis clinical strain with a defect in ESX-1 secretion induces minimal host immune responses and pathology Article Snippet: All M.tb strains used in this study were grown at 37 °C for 3–4 weeks either on solid Article Title: Rifampicin and isoniazid resistance not promote fluoroquinolone resistance in Mycobacterium smegmatis Article Snippet: Spontaneous FQ-resistant mutant colonies were selected on Article Title: Experimental Model of Tuberculosis in the Domestic Goat after Endobronchial Infection with Mycobacterium caprae Article Snippet: The inoculum was titrated after the inoculation in duplicate by 10-fold serial dilution in Article Title: The Mycobacterium tuberculosis β-oxidation genes echA5 and fadB3 are dispensable for growth in vitro and in vivo Article Snippet: Intracellular survival and growth was assessed by lysis of the monolayers by the addition of water followed by a 30 min incubation at Article Title: Identification of β-Lactams Active against Mycobacterium tuberculosis by a Consortium of Pharmaceutical Companies and Academic Institutions Article Snippet: To plate for CFU, lung homogenates were thawed, and bacilli were enumerated on Incubation:Article Title: Comparison of MALDI-TOF MS With HPLC and Nucleic Acid Sequencing for the Identification of Mycobacterium Species in Cultures Using Solid Medium and Broth Article Snippet: Objectives: The genus Mycobacterium contains over 150 species including pathogenic and nonpathogenic strains.. Accurate species level identification can aid in differentiating environmental contamination from true infection, and also can aid in selection of antimicrobial therapy.. Methods: We evaluated the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the routine identification of clinical isolates of mycobacteria using 2 commercially available spectral reference libraries, and also assessed the impact of mycobacterial culture using solid medium and broth on MALDI-TOF MS–based identification. Article Title: Azide-alkyne cycloaddition en route towards 1H-1,2,3-triazole-tethered β-lactam-ferrocene and β-lactam-ferrocenylchalcone conjugates: synthesis and in vitro anti-tubercular evaluation. Article Snippet: A diverse range of triazoles were prepared following well established, Cu-mediated azide–alkyne cycloaddition reactions with the aim of probing the anti-tubercular structure–activity relationships (SAR) within the β-lactam–ferrocene–triazole conjugate family.. The anti-tubercular evaluation studies of the synthesized conjugates revealed that none of the scaffolds exhibited any activity that restricted mycobacterial growth even at high doses.. The introduction of various substituents onto the N-1 of the β-lactam ring, introducing monoor di-ferrocenylchalcone substituents at the C-3 position as well as introducing a spacer of varying chain length failed to produce any significant enhancement in the activity profiles. Article Title: An attenuated Mycobacterium tuberculosis clinical strain with a defect in ESX-1 secretion induces minimal host immune responses and pathology Article Snippet: All M.tb strains used in this study were grown at 37 °C for 3–4 weeks either on solid Article Title: Rifampicin and isoniazid resistance not promote fluoroquinolone resistance in Mycobacterium smegmatis Article Snippet: Spontaneous FQ-resistant mutant colonies were selected on Article Title: Experimental Model of Tuberculosis in the Domestic Goat after Endobronchial Infection with Mycobacterium caprae Article Snippet: The inoculum was titrated after the inoculation in duplicate by 10-fold serial dilution in Article Title: The Mycobacterium tuberculosis β-oxidation genes echA5 and fadB3 are dispensable for growth in vitro and in vivo Article Snippet: Intracellular survival and growth was assessed by lysis of the monolayers by the addition of water followed by a 30 min incubation at Article Title: Identification of β-Lactams Active against Mycobacterium tuberculosis by a Consortium of Pharmaceutical Companies and Academic Institutions Article Snippet: To plate for CFU, lung homogenates were thawed, and bacilli were enumerated on Serial Dilution:Article Title: Comparison of MALDI-TOF MS With HPLC and Nucleic Acid Sequencing for the Identification of Mycobacterium Species in Cultures Using Solid Medium and Broth Article Snippet: Objectives: The genus Mycobacterium contains over 150 species including pathogenic and nonpathogenic strains.. Accurate species level identification can aid in differentiating environmental contamination from true infection, and also can aid in selection of antimicrobial therapy.. Methods: We evaluated the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the routine identification of clinical isolates of mycobacteria using 2 commercially available spectral reference libraries, and also assessed the impact of mycobacterial culture using solid medium and broth on MALDI-TOF MS–based identification. Article Title: Azide-alkyne cycloaddition en route towards 1H-1,2,3-triazole-tethered β-lactam-ferrocene and β-lactam-ferrocenylchalcone conjugates: synthesis and in vitro anti-tubercular evaluation. Article Snippet: A diverse range of triazoles were prepared following well established, Cu-mediated azide–alkyne cycloaddition reactions with the aim of probing the anti-tubercular structure–activity relationships (SAR) within the β-lactam–ferrocene–triazole conjugate family.. The anti-tubercular evaluation studies of the synthesized conjugates revealed that none of the scaffolds exhibited any activity that restricted mycobacterial growth even at high doses.. The introduction of various substituents onto the N-1 of the β-lactam ring, introducing monoor di-ferrocenylchalcone substituents at the C-3 position as well as introducing a spacer of varying chain length failed to produce any significant enhancement in the activity profiles. Article Title: An attenuated Mycobacterium tuberculosis clinical strain with a defect in ESX-1 secretion induces minimal host immune responses and pathology Article Snippet: All M.tb strains used in this study were grown at 37 °C for 3–4 weeks either on solid Article Title: Rifampicin and isoniazid resistance not promote fluoroquinolone resistance in Mycobacterium smegmatis Article Snippet: Spontaneous FQ-resistant mutant colonies were selected on Article Title: Experimental Model of Tuberculosis in the Domestic Goat after Endobronchial Infection with Mycobacterium caprae Article Snippet: The inoculum was titrated after the inoculation in duplicate by 10-fold serial dilution in Article Title: The Mycobacterium tuberculosis β-oxidation genes echA5 and fadB3 are dispensable for growth in vitro and in vivo Article Snippet: Intracellular survival and growth was assessed by lysis of the monolayers by the addition of water followed by a 30 min incubation at Article Title: Identification of β-Lactams Active against Mycobacterium tuberculosis by a Consortium of Pharmaceutical Companies and Academic Institutions Article Snippet: To plate for CFU, lung homogenates were thawed, and bacilli were enumerated on |
